Characterization and Quantification using LC-MS/MS: The Synergy Bioscience Advantage in Protein Analysis

In today’s fast-evolving pharmaceutical landscape, understanding complex biomolecules is paramount. The intricacies of protein structure and function can often be the linchpin determining the success of a new therapeutic or the precision of a diagnostic tool. This is where LC-MS/MS, or Liquid Chromatography-Tandem Mass Spectrometry, steps in as a revolutionary tool. For industry professionals well-acquainted with LC-MS, the technology’s possibilities are transformative.


Liquid Chromatography-Tandem Mass Spectrometry combines two potent techniques:

  • Liquid Chromatography (LC) separates compounds in a mixture, streamlining intricate molecular traffic.
  • Tandem Mass Spectrometry (MS/MS) subsequently identifies and provides detailed structural information about each separated molecule, revealing its nuanced features with incredible precision.

For protein analysis, LC-MS/MS offers:

  • Detailed Structural Insights: From amino acid sequences to three-dimensional conformations, LC-MS/MS provides a comprehensive view of therapeutic protein structures.
  • Quantification: Not just identifying, but accurately quantifying therapeutic protein concentrations in samples, pivotal for many biopharmaceutical applications.

The benefits of using LC-MS/MS can be summarized in:

  • Precision & Accuracy: Unparalleled in its precision, LC-MS/MS identifies even the most closely related proteins distinctly and quantifies them with impeccable accuracy.
  • Efficiency: Traditional methods pale in speed when compared to LC-MS/MS, propelling research into fast-track mode.
  • Versatility: LC-MS/MS is a multifaceted tool, essential across diverse scientific arenas.

The Synergy Bioscience Advantage:

to pharmaceutical executives and product development professionals: we all agree that expertise is non-negotiable. This is where Synergy Bioscience showcases its mastery. This can be highlighted in the following:

  • Extensive Project Handling: Be it extractable/leachable studies or reference standard/drug substance characterization, Synergy Bioscience stands ready.
  • Structural Insights: Delving into the structural elucidation of target/lead compounds and unknowns? We’ve got you covered.
  • Synthesis & Purification: Whether it’s the synthesis and purification of impurities and degradation products or amino acid analysis, our expertise is unparalleled.
  • Advanced Analyses: From peptide mapping, glycan analysis, and enantiomeric studies to structural characterization of protein variants and isoforms, our capabilities are both vast and deep.

In Conclusion, pharmaceutical and biological product development and approvals by regulatory bodies hinge on details. Tools like LC-MS/MS, when wielded by experts like those at Synergy Bioscience, pave the way for groundbreaking discoveries. For pharmaceutical companies and seasoned LC-MS professionals, partnering with a leader like Synergy Bioscience means harnessing the combined power of state-of-the-art technology and unparalleled expertise. Dive into the future of protein analysis with us; where precision meets potential.